A Robotics-Based Behavioral Paradigm to Measure Anxiety-Related Responses in Zebrafish
نویسندگان
چکیده
Zebrafish are gaining momentum as a laboratory animal species for the study of anxiety-related disorders in translational research, whereby they serve a fundamental complement to laboratory rodents. Several anxiety-related behavioral paradigms, which rest upon the presentation of live predatorial stimuli, may yield inconsistent results due to fatigue, habituation, or idiosyncratic responses exhibited by the stimulus itself. To overcome these limitations, we designed and manufactured a fully controllable robot inspired by a natural aquatic predator (Indian leaf fish, Nandus nandus) of zebrafish. We report that this robot elicits aversive antipredatorial reactions in a preference test and that data obtained therein correlate with data observed in traditional anxiety- and fear-related tests (light/dark preference and shelter-seeking). Finally, ethanol administration (0.25; 0.50; 1.00%) exerts anxiolytic effects, thus supporting the view that robotic stimuli can be used in the analysis of anxiety-related behaviors in zebrafish.
منابع مشابه
Understanding behavioral and physiological phenotypes of stress and anxiety in zebrafish.
The zebrafish (Danio rerio) is emerging as a promising model organism for experimental studies of stress and anxiety. Here we further validate zebrafish models of stress by analyzing how environmental and pharmacological manipulations affect their behavioral and physiological phenotypes. Experimental manipulations included exposure to alarm pheromone, chronic exposure to fluoxetine, acute expos...
متن کاملChronic Unpredictable Stress (CUS)-Induced Anxiety and Related Mood Disorders in a Zebrafish Model: Altered Brain Proteome Profile Implicates Mitochondrial Dysfunction
Anxiety and depression are major chronic mood disorders, and the etiopathology for each appears to be repeated exposure to diverse unpredictable stress factors. Most of the studies on anxiety and related mood disorders are performed in rodents, and a good model is chronic unpredictable stress (CUS). In this study, we have attempted to understand the molecular basis of the neuroglial and behavio...
متن کاملInformation Flow in Animal-Robot Interactions
The nonverbal transmission of information between social animals is a primary driving force behind their actions and, therefore, an important quantity to measure in animal behavior studies. Despite its key role in social behavior, the flow of information has only been inferred by correlating the actions of individuals with a simplifying assumption of linearity. In this paper, we leverage inform...
متن کاملComparison the Effectiveness of Cognitive Behavioral therapy focused on Insomnia with Mindfulness Based Cognitive therapy and Fordyce Happiness training on Pain Anxiety in Women with Migraine Headache.
Background: Pain anxiety is one of the most common psychological disorders in patients with migraine headaches that affects the response to treatment. This study aimed to comparison the effectiveness of cognitive behavioral therapy focused on Insomnia with mindfulness based cognitive therapy and Fordyce happiness training on pain anxiety in women with migraine headache Methods: The present ...
متن کاملNeurotranscriptome profiles of multiple zebrafish strains
Behavioral displays or physiological responses are often influenced by intrinsic and extrinsic mechanisms in the context of the organism's evolutionary history. Understanding differences in transcriptome profiles can give insight into adaptive or pathological responses. We utilize high throughput sequencing (RNA-sequencing) to characterize the neurotranscriptome profiles in both males and femal...
متن کامل